Te Whakatau i te Ihirangi Waikawa Oxalic i roto i ngā Hua me ngā Huawhenua mā te Chromatography Wai Mahi-Teitei (HPLC)
1. Te Kaupapa Whakamātautau me te Hoahoa Tikanga
Hei waikawa rauropi noa i roto i ngā hua me ngā huawhenua, ka pā tika te nui o te waikawa oxalic ki te reka me te uara kai o te kai. Ka whakamahia e tēnei whakamātautau te chromatography wai mahi-teitei-wāhanga-hurihuri (RP-HPLC). I raro i ngā āhuatanga wāhanga neke waikawa, ka wehea te waikawa oxalic mai i ngā matū whakararuraru mā te whakamahi i te pou chromatography C18. Ka whakamahia he taputapu kite ultraviolet kua whakatakotoria ki te 210 nm mō te tātari tauanga, i runga i ngā āhuatanga mimiti UV o ngā rōpū carboxyl i roto i ngā ngota waikawa oxalic.
2. Te Piko Paerewa me te Whakarite Tauira
Ka whakamahia he tikanga whakakōkī piki me te heke hei whakarite i ngā otinga paerewa: paunatia kia tika te 25.0 mg o te waikawa oxalic dihydrate ka whakakōkī kia 25 mL ki te wai tino parakore hei whiwhi i te otinga rakau 1 mg/mL. Ka whakakōkīhia tēnei kia rite ki te raupapa o ngā otinga paerewa i te 50, 100, 200, 400, me te 800 µg/mL, me ia kukū ka werohia kia toru ngā wā.
Mō te whakarite tauira, ka whakamahia te tangohanga āwhina-ngaruiti: paunatia te 5.00 karamu o te ranunga huarākau/huawhenua, tāpirihia he 10 mL o te otinga waikawa hauwai 0.1 mol/L, whakamahanahia ki te ngaruiti i te 60°C mō te 10 meneti, tātarihia mā te tātari kiriuhi 0.45 µm, ka kohia te tātari hei whakamātautau.
3. Te Arotau i ngā Āhuatanga Chromatographic
Ko te wāhanga nekeneke he pūnaha 0.01 mol/L pāhare pākawa dihydrogen phosphate (pH 2.5)–acetonitrile (95:5), me te tere rere o te 0.8 mL/min me te pāmahana pou o te 35°C. Mā te whakatikatika i te ōwehenga acetonitrile e whakaatu ana ina neke atu te wāhanga waro i te 10%, ka poto te wā pupuri o te waikawa oxalic ki raro iho i te 3 meneti, engari ka puta te tihi o te tailing. I raro i ngā tikanga whakamutunga kua arotauhia, ko te wā pupuri o te waikawa oxalic he 4.2 meneti, ka tutuki te wehenga katoa mai i te waikawa citric me te waikawa malic e tata ana (taumira > 1.5).
4. Whakamana Tikanga
E whakaatu ana te manatoko whānuitanga rārangi i te whanaungatanga rārangi pai i waenga i te horahanga tihi me te kukū i te whānuitanga o te 10–1000 µg/mL (R² = 0.9993). Ko te rohe o te kitenga (LOD), i whakatauhia e te tikanga ōwehenga tohu-ki-haruru, ko te 0.5 µg/mL. I roto i ngā whakamātautau whakahokinga, ko te RSD mō ngā whakataunga tukurua e ono o te tauira pīnihi kotahi ko te 1.8%. Ko ngā whakamātautau whakaora pūwero i ngā taumata e toru (80%, 100%, me te 120%) ka puta he whakaora toharite o te 98.2%, 102.4%, me te 97.8%, ia, e tutuki ana i ngā whakaritenga mō te tātari tauanga.
5. Te Tātari i ngā Tauira Tuturu
E ono ngā hua me ngā huawhenua e wātea ana i te hokohoko i whakamatautauria: te pīnihi (356 ± 12 mg/100 g), te herewi (215 ± 9 mg/100 g), te tōmato (18 ± 2 mg/100 g), te āporo (6 ± 1 mg/100 g), te panana (kāore i kitea), me te pūrokoro (89 ± 5 mg/100 g). Mā te whakataurite ki te tikanga paerewa ā-motu (GB 5009.277-2016) e whakaatu ana ko te rerekētanga whanaunga i waenga i ngā tikanga e rua he iti iho i te 5%, e whakamana ana i te pono o tēnei tikanga. Ina koa, he nui ake te aro o te tikanga HPLC i ngā tikanga ine ine tuku iho ina tātarihia ana ngā tauira iti-ipurangi.
6. Ngā Tuhipoka Matua
Kia tino aroturukihia te uara pH i te wā e tukatukahia ana te tauira. Mena ka neke atu te pH o te tangohanga i te 3, ka iti ake ngā hua whakamātautau o te maturuturunga o te konupūmā oxalate. Me whakarite kia hou tonu te wāhanga neke i ia rā kia kore ai e maturuturu te tote me te aukati i te pou. I muri i ia werohanga 20, horoia te pou ki te wai acetonitrile (30:70) mō te 30 meneti hei ārai i te hekenga o te whai huatanga o te pou. Mō ngā tauira kei roto he tae (hei tauira, te kāpeti papura), e taunakihia ana kia tāpirihia he taahiraa purenga tangohanga totoka i mua i te werohanga.
7. Ngā Tohutohu mō te Whakamahinga me te Whakawhanui
Ka taea te whakawhānui ake i tēnei tikanga ki te kimi i te waikawa oxalic i roto i ngā tauira koiora pēnei i te mimi me te toto. Mā te whakarerekē i te hanganga o te wāhanga neke (hei tauira, te tāpiri i te tetrabutylammonium hydroxide), ka taea te whakatau i te waikawa oxalic me ōna matū whakaputa i te wā kotahi. Mā te whakamahi tahi me te taputapu tirotiro ira papatipu, ka taea te whakatū i tētahi tikanga kimi tohu tika ake. I roto i te umanga tukatuka kai, ka taea e tēnei tikanga te whakarato tautoko raraunga nui mō te whiriwhiri i ngā momo waikawa oxalic iti me te arotau i ngā tukanga tunu kai.
Zibo Anhao Chemical Co., Ltd. – Waikawa Oxalic
He kaiwhakarato pono, he kaihokohoko hoki a Zibo Anhao Chemical Co., Ltd. o te waikawa oxalic kounga teitei (tohu ahumahi, 99.6% min) mai i Shandong, Haina. He totoka tioata mā tō mātou waikawa oxalic, e mōhiotia ana ko te waikawa ethanedioic, ko tōna tauira ngota C₂H₂O₄. He tino parakore, he iti ngā poke, ā, he pumau te kounga puta noa i ngā puranga, e tutuki ana i ngā hiahia o ngā ahumahi kakahu, hiako, whakarewa, tukatuka whenua onge, horoi matū, me te hanga.
Ka tukuna e mātou te waikawa oxalic korewaihā me te waikawa oxalic dihydrate, e wātea ana i roto i ngā putea 25kg, 50kg, me ngā putea papatipu 1000kg. Mā ngā tikanga QC tino pakari, ngā pūrongo whakamātautau katoa, me ngā mahere tuku ngāwari, ka whakarite mātou i te tuku pumau me te mahi pumau mō ngā kaihoko o te ao. Kua whānuitia ā mātou hua ki te kaweake ki te Tonga-mā-rāwhiti o Āhia, ki Ūropi, ki Āwherika, me Amerika, e mōhiotia ana mō te kounga pono me te utu whakataetae.
Kōwhiria a Zibo Anhao Chemical mō te tuku waikawa oxalic pono me te ratonga matū kotahi-mutu ngaio.
Kōwhiria te Zibo Anhao Chemical mō te waikawa oxalic tino parakore e whakarato ana i te mahi pumau me te uara mō tō pakihi. Whakapā mai ki a mātou mō ngā ota nui, ngā whakaritenga hangarau rānei: info@anhaochemical.com
